The Vildebeest was purchased in moderately large numbers by the Royal Air Force from 1931 and used as a torpedo bomber. It entered service with No. 100 Squadron at RAF Donibristle in Scotland in October 1932, replacing the Hawker Horsley. Four frontline torpedo-bomber squadrons were equipped with the Vildebeest, two at Singapore (100 Squadron, which moved from the United Kingdom in 1933 and 36 Squadron, which replaced its Horsleys in 1935), and two more in the United Kingdom.
The Vincent entered service with No. 84 Squadron RAF at Shaibah, Iraq in December 1934, re-equipping General Purpose squadrons throughout the Middle East and Africa. By 1937, it equipped six squadrons in Iraq, Aden, Kenya, Sudan, and Egypt.
At the outbreak of the Second World War, 101 Vildebeests were still in service with the RAF. The two British-based squadrons flew coastal patrol and convoy escort missions until 1940 when their Vildebeests were replaced by the Bristol Beaufort. The two Singapore-based squadrons were still waiting for their Beauforts when Japan invaded Malaya in December 1941 and the obsolete biplanes had to be deployed against the Japanese attackers, making an abortive torpedo attack on a Japanese cruiser off Kota Bharu on 8 December.
The Vildebeests continued to attack the Japanese as their forces advanced down Malaya, sustaining heavy losses from Japanese fighters, particularly when no fighter cover could be provided. On 26 January 1942, the Japanese landed at Endau, 250 miles from Singapore, and 12 Vildebeests of 100 and 36 Squadrons were sent to attack the landings. Despite an escort of Brewster Buffalo and Hawker Hurricane fighters, five Vildebeests were lost. The attack was repeated later that day by eight Vildebeests of 36 Squadron and three Fairey Albacores, resulting in six more Vildebeests and two Albacores being shot down. The surviving Vildebeests were withdrawn to Java on 31 January and attacked another Japanese landing force off Rembang, claiming eight ships sunk but sustaining further losses. The final two Vildebeests of 36 Squadron attempted to escape to Burma on 6 March but were lost over Sumatra. The last Vildebeests in RAF service, operated by 273 Squadron at Ceylon were retired in March 1942.
While the Vincent had started to be replaced by more modern aircraft such as the Vickers Wellesley and Bristol Blenheim bombers, 84 remained in service with the RAF on the outbreak of the Second World War. Vincents were used for bombing missions against Italian forces in the East African Campaign and for coastal patrols from Aden, one attacking the Italian submarine Galileo Galilei. Other Vincents bombed Iraqi forces during the Anglo-Iraqi War of 1941. The last frontline Vincents retired in January 1943, with the type continuing in second line service (which included pesticide spraying against locusts in Iran) until 1944.
The Vildebeest was ordered by the Spanish Republic in 1932 and licence production of 25 Vildebeest for the Spanish Republican Navy was undertaken in Spain by CASA most receiving the Hispano-Suiza HS 600 inline engine, though some other engines were also used. Around 20 survived to fight with the Spanish Republican Air Force on the loyalist side of the Spanish Civil War, some equipped with floats.
A Vildebeest was the first victim of Francoist ace Joaquin Garcia-Morato.
12 Vildebeests were purchased by the Royal New Zealand Air Force in 1935 for coastal defence, with a further 27 acquired from RAF stocks in 1940-41. In addition, 60 or 62 of these machines, (depending on source), were passed on to the RNZAF.
New Zealand Vildebeests were also used for photo mapping. A few were used for maritime patrols against German surface raiders, and Japanese submarines, (a handful were based in Fiji in December 1941), but the main wartime role of the New Zealand aircraft was as particularly unwieldy pilot trainers, until replaced by North American Harvards in 1942.